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dc.contributor.authorLeal, Ernesto Cesar Pinto Jreng
dc.contributor.authorde Almeida, Patríciaeng
dc.contributor.authorTomazoni, Shaiane Silvaeng
dc.contributor.authorde Carvalho, Paulo de Tarso Camilloeng
dc.contributor.authorLopes-Martins, Rodrigo Alvaro Brandaoeng
dc.contributor.authorFrigo, Lúcioeng
dc.contributor.authorJoensen, Joneng
dc.contributor.authorJohnson, Mark I.eng
dc.contributor.authorBjordal, Jan Magnuseng
dc.date.accessioned2015-03-30T08:18:24Z
dc.date.available2015-03-30T08:18:24Z
dc.date.issued2014-03-05eng
dc.identifier.issn1932-6203eng
dc.identifier.urihttp://hdl.handle.net/1956/9694
dc.description.abstract<p>Aim: To evaluate the effects of preventive treatment with low-level laser therapy (LLLT) on progression of dystrophy in mdx mice.</p> <p>Methods: Ten animals were randomly divided into 2 experimental groups treated with superpulsed LLLT (904 nm, 15 mW, 700 Hz, 1 J) or placebo-LLLT at one point overlying the tibialis anterior muscle (bilaterally) 5 times per week for 14 weeks (from 6th to 20th week of age). Morphological changes, creatine kinase (CK) activity and mRNA gene expression were assessed in animals at 20th week of age.</p> <p>Results: Animals treated with LLLT showed very few morphological changes in skeletal muscle, with less atrophy and fibrosis than animals treated with placebo-LLLT. CK was significantly lower (p = 0.0203) in animals treated with LLLT (864.70 U.l−1, SEM 226.10) than placebo (1708.00 U.l−1, SEM 184.60). mRNA gene expression of inflammatory markers was significantly decreased by treatment with LLLT (p<0.05): TNF-α (placebo-control = 0.51 µg/µl [SEM 0.12], - LLLT = 0.048 µg/µl [SEM 0.01]), IL-1β (placebo-control = 2.292 µg/µl [SEM 0.74], - LLLT = 0.12 µg/µl [SEM 0.03]), IL-6 (placebo-control = 3.946 µg/µl [SEM 0.98], - LLLT = 0.854 µg/µl [SEM 0.33]), IL-10 (placebo-control = 1.116 µg/µl [SEM 0.22], - LLLT = 0.352 µg/µl [SEM 0.15]), and COX-2 (placebo-control = 4.984 µg/µl [SEM 1.18], LLLT = 1.470 µg/µl [SEM 0.73]).</p> <p> Conclusion: Irradiation of superpulsed LLLT on successive days five times per week for 14 weeks decreased morphological changes, skeletal muscle damage and inflammation in mdx mice. This indicates that LLLT has potential to decrease progression of Duchenne muscular dystrophy. </p>en_US
dc.language.isoengeng
dc.publisherPLoSeng
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0eng
dc.titleSuperpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progressioneng
dc.typeJournal articleeng
dc.subject.nsiVDP::Medisinske fag: 700::Klinisk medisinske fag: 750::Fysikalsk medisin og rehabilitering: 764nob
dc.subject.nsiVDP::Medisinske fag: 700::Klinisk medisinske fag: 750::Radiologi og bildediagnostikk: 763nob
dc.subject.nsiVDP::Medical sciences: 700::Clinical medical sciences: 750::Physical medicine and rehabilitation: 764eng
dc.subject.nsiVDP::Medical sciences: 700::Clinical medical sciences: 750::Radiology and diagnostic imaging: 763eng
dc.date.updated2015-03-03T16:15:45Zen_US
dc.rights.holderCopyright 2014 Leal-Junior et al
dc.type.versionpublishedVersioneng
bora.peerreviewedPeer reviewedeng
bora.journalTitlePLoS ONEeng
bibo.volume9eng
bibo.issue3eng
bibo.numbere89453eng
bibo.doihttp://dx.doi.org/10.1371/journal.pone.0089453eng
bora.accessRightsinfo:eu-repo/semantics/openAccesseng
dc.identifier.cristinID1156167eng
dc.identifier.doi10.1371/journal.pone.0089453


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