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dc.contributor.authorLydvo, Steffeneng
dc.date.accessioned2015-09-23T15:42:03Z
dc.date.available2015-09-23T15:42:03Z
dc.date.issued2015-06-01
dc.date.submitted2015-06-01eng
dc.identifier.urihttp://hdl.handle.net/1956/10519
dc.description.abstractMarine subsurface sediments are large and ecologically significant microbial habitats. The archaeal phylum Lokiarchaeota is a group of organisms commonly found in these sediments. Their metabolism is unknown, but based on several indirect lines of evidence, it has been suggested that they are dissimilatory iron and/or manganese reducers, oxidising organic carbon using ferric iron [Fe(III)] and manganese [Mn(IV)] as electron acceptors. This study aims to further investigate these claims using a two-pronged approach: Firstly, to attempt to enrich Lokiarchaeota in vitro, and monitor the growth using molecular methods; secondly, to correlate Lokiarchaeota abundance data from quantitative polymerase chain reaction (qPCR) with data on iron and manganese concentration in the porewater of a long sediment core from the Arctic Mid-Ocean Ridge. The results of the study are largely inconclusive, but some evidence that support the hypothesis was found. 16S rRNA gene community profiles suggest that Lokiarchaeota might have grown in one of the enrichments containing amorphous Fe(III)-oxide and pyruvate, and a possible correlation between dissolved Fe(II) and Lokiarchaeota abundance was found in the sediment core.eng
dc.format.extent5182331 byteseng
dc.format.mimetypeapplication/pdfeng
dc.language.isoengeng
dc.publisherThe University of Bergeneng
dc.subjectlokiarchaeotaeng
dc.subjectiron reductioneng
dc.subjectmarine sedimentseng
dc.subjectjernreduksjonnob
dc.subjectmarine sedimenterNOB
dc.titleInvestigation of the putative iron reducing capabilities of Lokiarchaeotaeng
dc.typeMaster thesiseng
dc.type.degreeMaster i Biologieng
dc.type.courseBIO399eng
dc.subject.archivecodeMastergradeng
dc.subject.nus751999eng
dc.type.programMAMN-BIOeng
dc.rights.holderCopyright the Author. All rights reservedeng
bora.peerreviewedNot peer reviewedeng
bibo.doi
noa.realfagstermhttp://data.ub.uio.no/realfagstermer/c012415


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